TABLE 1

Positive ion LC/MS/MS data of metabolites of pioglitazone in freshly isolated human, rat, and monkey hepatocytes

Metabolites	Retention Time	[M + H]⁺	Product Ions	Freshly Isolated Hepatocytes
Metabolites	Retention Time	[M + H]⁺	Product Ions	Human	Rat	Monkey
	min	m/z	m/z
M1	7.8	361	119, 134, 254 (B), 280, 297	+	+	+
M2	12.2	551	134, 286, 332 (B), 358, 375, 417	+	+	+
M3	13.2	377	119, 134, 254 (B), 280, 297	+	+	+
M4	14.5	373	132, 150 (B), 240, 256	+	N.D.	+
M5	15.0	373	132, 135, 150 (B), 238, 355	+	+	+
M6	15.2	387	146, 164 (B)	+	+	+
M7	16.8	533	134, 357 (B)	+	+	+
M8	17.8	345	119, 134 (B), 240, 254, 280, 328	+	N.D.	+
M9	18.2	373	119, 134, 150, 240, 268 (B), 284, 313, 355	+	+	+
M10	19.4	373	133, 150, 210 (B), 222, 238, 239, 284, 355	+	+	+
M11	23.3	346	119, 134 (B), 254, 270, 286,	+	+	+
M12	23.9	355	132 (B), 210, 238, 239, 284	+	N.D.	+
M13	30.1	371	106, 148 (B), 254	+	+	+