Effect of 5-azaCyd and 5-aza-2′-dCyd on the sensitivity of hCNT3-expressing MDCK cells

MDCK-Co and MDCK-CNT3 cells were incubated with different concentrations of 5-azaCyd and 5-aza-2′-dCyd for 72 h, and the cell viability was determined by AlamarBlue assays (n = 8).

Nucleoside AnalogIC50aRelative Sensitivityb
5-azaCyd54 ± 50.8 ± 0.0767.5
5-aza-2′-dCyd331 ± 4825 ± 413.2
  • a IC50 is the nucleoside analog concentration required to reduce cell survival by 50%.

  • b The relative sensitivity is the IC50 for nucleoside analog treatment in MDCK-Co cells divided by the IC50 in MDCK-CNT3 cells.