Aclidinium hydrolysis and BChE activities in different human subcellular fractions
The net enzymatic hydrolysis results are expressed as the mean of duplicate measurements. BChE activities were determined as reported in the experimental section and expressed as the mean of three measurements ± S.D. unless indicated otherwise.
| Tissue | Fraction | Source/Batch | Net Enzymatic Aclidinium Hydrolysis | BChE Activity |
|---|---|---|---|---|
| pmol alcohol/min/mg protein | nmol thiocholine/min/mg protein | |||
| Blood | Plasma | Almirall, S.A./NA | 93.2 | 50.9 ± 16.0a |
| Red blood cells | Almirall, S.A./NA | 1.1 | 1.9 ± 0.1 | |
| Liver | Microsomal | XenoTech,/0210002 | 19.0 | 7.0 ± 0.1 |
| S9 | Human Biologics International/1.0 | 42.0 | 4.2 ± 0.1 | |
| Lung | Microsomal | XenoTech,/0210354 | 38.0 | 15.5 ± 0.2 |
| S9 | XenoTech,/0210352 | 54.0 | 13.3 ± 0.2 |
NA, not applicable.
↵a Mean value ± S.D. (n = 6).