TABLE 2

Aclidinium hydrolysis in recombinant human AChE, purified human BChE, and human albumin

Incubations of aclidinium (5 μM) were conducted with pure enzymes containing human serum albumin (0.25 mg/ml) for 30 min. Mean values are from duplicate measurements (<10% variance).

	Enzyme Concentration	Rate
	mU/ml^{^a}	pmol acid/min/ml
Phosphate buffer, pH 7.4	NA	29.7
Human serum albumin	0.25 mg/ml	30.2
AChE	1	19.0
	10	22.7
	100	26.3
	1000	30.7
BChE	1	31.0
	10	33.0
	100	53.8
	1000	180.8

NA, not applicable.
↵a Relative units. One unit of AChE or BChE hydrolyzes 1.0 μmol of acetylcholine or butyrylcholine to choline and acetate/butyrate per minute at pH 8.0 and 37°C.