TABLE 3

Solubility (μg/ml) of SGX523 versus metabolite M11

Compound was accurately weighted, and 1 ml of the appropriate matrix was added. Vials were capped and incubated at 37°C overnight. Samples were then centrifuged and filtered using a 0.45-μm filter, and the concentrations of SGX523 and M11 in the supernatant were determined by LC/MS/MS analysis.

MatrixCompound
SGX523M11 Metabolite
μg/mlμg/ml
Water (pH 6.8)5.40.13
Buffer (pH 7.4)4.00.10
0.1 N HCl (pH 1.0)27340.25
Cynomolgus monkey urine (pH 8.4)130.37