TABLE 2

K_d and B_max for specific [³H]NMS binding in the bladder and submaxillary gland of rats after the oral administration of propiverine (24.8–248 μmol/kg)

Values are the mean ± S.E. for four or five rats. Rats received 24.8, 74.3, and 248 μmol/kg propiverine orally and were sacrificed 1 to 12 h later. Specific binding of [³H]NMS (0.03–1.5 nM) in the rat bladder was measured. Values in parentheses represent the fold increase in K_d values relative to controls.

Organs	Dose	Time	K_d	B_max
	μmol/kg	h	pM	fmol/mg protein
Bladder	Control		200 ± 15	94 ± 6
	24.8	1	251 ± 20	97 ± 3
		3	275 ± 27 (1.38)^*	99 ± 4
		6	239 ± 7	99 ± 6
	74.3	1	302 ± 30 (1.51)^*	102 ± 4
		3	352 ± 27 (1.76)^**	112 ± 8
		6	301 ± 7 (1.51)^*	106 ± 6
		12	281 ± 9 (1.41)^*	107 ± 6
	248	1	277 ± 21 (1.39)^*	106 ± 5
		3	343 ± 16 (1.72)^**	104 ± 5
		6	351 ± 20 (1.76)^**	102 ± 14
		12	290 ± 13 (1.45)^**	103 ± 4
Submaxillary gland	Control		142 ± 2	97 ± 4
	24.8	1	162 ± 18	86 ± 6
		3	173 ± 17	87 ± 8
		6	157 ± 17	85 ± 2
	74.3	1	210 ± 16 (1.48)^**	91 ± 3
		3	200 ± 19 (1.41)^**	73 ± 5^**
		6	179 ± 8	76 ± 2^**
		12	150 ± 6	93 ± 2
	248	1	185 ± 16	74 ± 4^**
		3	278 ± 19 (1.96)^**	76 ± 3^**
		6	273 ± 33 (1.92)^**	70 ± 4^**
		12	216 ± 15	93 ± 1

↵* P < 0.05 and
↵** P < 0.01, asterisks show a significant difference from the control values.