TABLE 1

Primer oligonucleotides used to clone the genes

The enzyme restriction sites for cloning purposes are indicated in capital letters. ATG translation start codon coding for the starting amino acid of the protein (Met) is indicated in capital letters.

OligonucleotidesSequences 5′ to 3′Fragment LengthTarget Reference cDNA
bp
CYP1A2 FP-KpnIgcaGGTACCgttggtacagATGgcatt1627NM_000761
CYP1A2 RP-KpnIaaaGGTACCgaacaagggctgagtc
CYP2C9 RP-EcoRIgGAATTCggcttcaATGgattctcttgtgg1485NM_000771
CYP2C9 FP-XbaIcgTCTAGActtcttcagacaggaatgaa

  • bp, basepair.