TABLE 4
Metabolite formation kinetic parameter values in Ad-P450 HepG2 cells
P450
Substrate
K
m
(or
S
50
)
K
si
n
V
max
CL
int
μ
M
pmol/(min
·
10
6
cells)
μ
l/(min
·
10
6
cells)
CYP1A2
Phenacetin
12.3 ± 2.6
448 ± 217
39.6 ± 24.5
CYP2C9
Tolbutamide
29.8 ± 3.1
7050 ± 4242
69.0 ± 4.7
2.34 ± 0.317
CYP3A4
Alprazolam
659 ± 560
a
1.6
2160 ± 1330
2.17 ± 0.683
b
CYP3A4
Midazolam
4.59 ± 2.47
242 ± 78
185 ± 45
50.4 ± 31.1
↵
a
S
50
value.
↵
b
CL
max
value.