TABLE 4

Metabolite formation kinetic parameter values in Ad-P450 HepG2 cells

P450SubstrateKm (or S50)KsinVmaxCLint
μMpmol/(min·106 cells)μl/(min·106 cells)
CYP1A2Phenacetin12.3 ± 2.6448 ± 21739.6 ± 24.5
CYP2C9Tolbutamide29.8 ± 3.17050 ± 424269.0 ± 4.72.34 ± 0.317
CYP3A4Alprazolam659 ± 560a1.62160 ± 13302.17 ± 0.683b
CYP3A4Midazolam4.59 ± 2.47242 ± 78185 ± 4550.4 ± 31.1

  • ↵a S50 value.

  • ↵b CLmax value.