TABLE 1

Comparison of kinetic parameters for DBZ metabolites formed in RLMs and HLMs

The quantity of each metabolite was represented by its area under the curve (AUC) on the chromatogram, because calibration was unnecessary for the purpose of this comparison. Values are means ± S.E. obtained from three independent experiments.

	K_m		V_max		V_max/K_m
	RLM	HLM	RLM	HLM	RLM	HLM
	μM		AUC/min/mg
M1	41.8 ± 1.3	24.7 ± 1.3^*	12.9 ± 0.3	0.63 ± 0.03^**	0.308 ± 0.015	0.026 ± 0.002^**
M2	47.9 ± 0.4	25.0 ± 1.3^*	7.1 ± 0.5	0.58 ± 0.02^**	0.149 ± 0.008	0.023 ± 0.002^**
M3	42.2 ± 0.3	19.6 ± 0.5^*	18.7 ± 1.9	1.89 ± 0.08^**	0.443 ± 0.042	0.096 ± 0.003^**
M4	34.2 ± 0.7	24.1 ± 0.8^*	41.9 ± 2.6	0.95 ± 0.06^**	1.227 ± 0.100	0.040 ± 0.001^**
M5	31.5 ± 1.6	23.2 ± 0.5^*	7.6 ± 0.5	0.97 ± 0.05^**	0.242 ± 0.003	0.042 ± 0.002^**

↵* p < 0.05.
↵** p < 0.01 versus the RLM values.