Oligonucleotide primer and probe sequences used for quantitative PCR
| Gene | Oligonucleotide Sequence (5′-3′) | Exon Location |
|---|---|---|
| CYP1A2 | ||
| Forward primer | CATCCCACAGGAGAAGATTGTCA | 3 |
| Reverse primer | TTCCTCTGTATCTCAGGCTTGGT | 4 |
| Probe: 6-FAM MGB | TGGAGCAGGATTTGAC | 3/4 |
| CYP2B6 | ||
| Forward primer | GCTGAACTTGTTCTACCAGACTTTTTC | 4 |
| Reverse primer | GAAAGTATTTCAAGAAGCCAGAGAAGAG | 5 |
| Probe: 6-FAM MGB | TGTATTCGGCCAGCTGT | 4/5 |
| CYP2C19 | ||
| Forward primer | GACTTTATTGATTGCTTCCTGATCAA | 5 |
| Reverse primer | GCAGTGATTACCAAGTTTTCAATAGTG | 6 |
| Probe: 6-FAM MGB | ATGGAGAAGGAAAAGCAA | 5/6 |
| CYP2D6 | ||
| Forward primer | CTCCTGCTCATGATCCTACATCC | 6 |
| Reverse primer | CGGGATGTCATATGGGTCACACC | 7 |
| Probe: VIC MGB | TGATTCATGAGGTGCAG | 7 |
| CYP3A4 | ||
| Forward primer | TGTCCTACCATAAGGGCTTTTGTAT | 2/3 |
| Reverse primer | TTCACTAGCACTGTTTTGATCATGTC | 4 |
| Probe: 6-FAM TAMRA | CTTTTATGATGGTCAACAGCCTGTGCTG | 4 |