TABLE 2

Kinetic parameters for phenacetin and APAP hydrolase activities by recombinant AADAC, CES1A1, and CES2

KmVmaxCLint
mMnmol/(min · mg)μl/(min · mg)
Phenacetin
    AADAC1.82 ± 0.02*6.03 ± 0.14*3.32 ± 0.05**
    CES1A10.27 ± 0.010.29 ± 0.021.09 ± 0.01
    CES20.30 ± 0.020.38 ± 0.031.25 ± 0.01
APAP
    AADAC32.2 ± 1.32.77 ± 0.160.07 ± 0.02*
    CES1A1N.A.N.A.0.01 ± 0.00
    CES2N.A.N.A.0.02 ± 0.00

  • N.A., not applicable.

  • ↵* P < 0.005 compared with CES1A1 and CES2.

  • ↵** P < 0.001 compared with CES1A1 and CES2.