TABLE 1

NMR determination of the concentration of M1 (isolated from microsomal incubations) produced from compound 1

Compound	Nominal (Weighed) Concentration	Mean Observed Concentration by NMR	%RSD	%Difference from Nominal Value
	mM
Compound 1	3.80	3.80	0.15	0.02
	2.50	2.48	0.09	0.80
	1.00	1.05	0.37	4.69
	0.50	0.50	0.08	0.11
	0.10	0.07	0.65	42.45
QC^{^a}	3.50	3.41	1.68	2.73
	2.00	2.12	0.33	5.77
	0.20	0.18	0.25	11.28
M1		1.72	0.16

↵a Another metabolite of compound 1, available as a synthetic standard, was used as a quality control to ensure that the NMR quantitation was verified by gravimetric determination. NMR analysis was conducted in triplicate.