Kinetic parameters for the formation of the 6′b-OH hydroxylated product by CYP102A1 mutants
| P450 Enzyme | Simvastatin | Lovastatin | ||||
|---|---|---|---|---|---|---|
| kcat | Km | kcat/Km | kcat | Km | kcat/Km | |
| min−1 | mM | min−1/μM | min−1 | mM | min−1/μM | |
| Mutant 16 | 10 ± 1 | 37 ± 6 | 0.27 ± 0.05 | 14 ± 1 | 44 ± 9 | 0.32 ± 0.07 |
| Mutant 17 | 15 ± 1 | 42 ± 6 | 0.36 ± 0.60 | 19 ± 2 | 41 ± 9 | 0.46 ± 0.11 |
| Human CYP3A4 | 6.6 ± 0.9 | 130 ± 30 | 0.051 ± 0.014 | 4.2 ± 0.5 | 54 ± 15 | 0.078 ± 0.024 |