IC50 values of pancreatic cancer cell lines to 5-FU, GEM, 6-TG, and 6-MP
Values were determined by crystal violet assays as described under Materials and Methods. At least five drug concentrations were used to determine IC50 values. Experiments at each concentration were carried out in triplicate, and the IC50 values were calculated by nonlinear regression from a sigmoidal concentration-response curve (Prism software 3.0). Data represent the mean ± S.E. of cumulated data of three independent experiments performed in triplicate (n = 9).
| Cell line | IC50 Value | |||
|---|---|---|---|---|
| 5-FU | GEM | 6-TG | 6-MP | |
| μM | nM | μM | ||
| T3M4 | 0.33 ± 0.06 | 1.50 ± 0.01 | 1.28 ± 0.03 | 2.63 ± 0.02 |
| Colo-357 | 2.05 ± 0.03 | 0.36 ± 0.01 | 2.09 ± 0.33 | 6.12 ± 0.07 |
| Patu-T | 2.08 ± 0.07 | 2.80 ± 0.03 | 3.43 ± 0.03 | 4.09 ± 0.04 |
| Patu-S | 2.93 ± 0.04 | 3.20 ± 0.03 | 2.21 ± 0.03 | 11.07 ± 0.07 |
| Aspc-1 | 2.97 ± 0.03 | 4.00 ± 0.04 | 2.80 ± 0.02 | 2.45 ± 0.01 |
| Panc-1 | 3.94 ± 0.03 | 1.00 ± 0.02 | 8.31 ± 0.27 | 6.39 ± 0.02 |
| Patu-02 | 3.95 ± 0.01 | 3.40 ± 0.03 | 2.26 ± 0.02 | 9.69 ± 0.04 |
| Dan-G | 4.38 ± 0.03 | 3.40 ± 0.02 | 4.32 ± 0.09 | 4.06 ± 0.01 |