Infusion scheme and resulting total plasma concentrations of morphine measured by LC-MS/MS and AMS
No significant differences were observed between the two methods when the same samples were compared.
| Steady-State Level | Number of Rats | Bolus Infusion (10 min) | Constant Infusion (110 min) | Target Conc. | Measured Plasma Conc.* LC-MS/MS | Measured Plasma Conc. AMS |
|---|---|---|---|---|---|---|
| n | mg · kg−1 · h−1 | mg · kg−1 · h−1 | ng · ml−1 | ng · ml−1 | ng · ml−1 | |
| Ultra Low low | 1 | 0.0156 | 0.00156 | 0.4 | 0.16 | |
| Very Low | 1 | 0.0624 | 0.0078 | 2 | 1.12 | 0.8 |
| Low | 7 | 0.4 | 0.04 | 10 | 8.5 ± 3.3 | 6.93 ± 2.07 |
| Medium | 6 | 1.56 | 0.20 | 50 | 47.2 ± 19 | 41.2 ± 15.80 |
| High | 6 | 7.80 | 0.98 | 250 | 208 ± 63 | 215 ± 60.5 |
↵* Each value is the mean of three measurements for each level for each rat.