Formation of ER-879123 after 60-min incubations of E7016 with dog and human liver S9 fractions under various incubation conditions
Data are means ± S.D. n = 3.
| Incubation Conditions | ER-879123 SRM (382 → 249) Peak Area Counts × 104 | |
|---|---|---|
| Dog | Human | |
| No cofactor | 0.061 ± 0.024 | 0.23 ± 0.03 |
| NAD+ | 103 ± 3 | 2.0 ± 0.1 |
| NAD+ and 4-methylpyrazole | 75 ± 4 | 1.4 ± 0.1 |
| NAD+ and methimazole | 104 ± 9 | 2.3 ± 0.1 |
| NAD+ and BNPP | 34 ± 2 | 1.5 ± 0.1 |
| NAD+ with heated liver S9 | 18 ± 2 | 1.6 ± 0.1 |
| NADP+ | 42 ± 1 | 1.3 ± 0.0 |