TABLE 3 Influence of rat albumin on brain tissue binding
Binding affinities to brain lipids [KD(lipid)] and RSA [KD(RSA)] were determined by TRANSIL experiments and the free fractions based on binding to lipids only [fu(lipid)]. The values for binding to lipids and brain tissue albumin [fu(lipid + RSA)] were calculated according to eq. 6. The free fraction determined by equilibrium dialysis [fu(ED)] was assessed using perfused rat brains. The difference in the unbound fraction between binding to lipids only and binding to lipids and brain tissue albumin is denoted by ΔRSA.