TABLE 1
qNMR internal standard and aSICCO linearity and dynamic range data of NBG (0.01–10 mM NBG in DMSO-d6)
| NBG Chemical Shift | |||||||
|---|---|---|---|---|---|---|---|
| 2.01 ppm (7′) | 3.78 ppm (4′) | 4.58 ppm (2′) | 7.61 ppm (3/5) | 8.18 ppm (2/6) | 8.37 ppm (5′) | 8.49 ppm (3′) | |
| Istd method | |||||||
| r2 | 1.000 | 0.999 | 1.000 | 1.000 | 1.000 | 1.000 | 1.000 |
| Slope | 0.098 | 0.15 | 0.050 | 0.101 | 0.106 | 0.051 | 0.051 |
| Intercept | 0.00 | −0.02 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| aSICCO | |||||||
| r2 | 1.000 | 1.000 | 1.000 | 1.000 | 1.000 | 1.000 | 1.000 |
| Slope | 0.59 | 0.61 | 1.2 | 0.60 | 0.67 | 1.3 | 1.3 |
| Intercept | 0.00 | 0.04 | 0.03 | −0.03 | 0.00 | 0.00 | 0.01 |
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