Experimental conditions in CYP2C19 enzyme activity and inhibition assays in a 150-μl incubation volume
| Condition | Value |
|---|---|
| Tris-HCl, pH 7.4 | 100 mM |
| Substrate (DBF) concentration | 1.0 μM (equals Km) |
| Enzyme (human recombinant CYP2C19) | 1.5 pmol |
| NADPH-regenerating system | 50 μl |
| Incubation time at 37°C | 30–60 min |
| Excitation/emission wavelengths (nm) | 485/535 |