TABLE 3

Signal/noise ratios of enzyme-catalyzed reactions

BlankaMeasurement
Immediately after IncubationAfter the First Measurementb
Fold difference between enzyme-catalyzed and blank samples
Blank I2.43.5
Blank II11.917.9
  • a Blank I: as enzyme-catalyzed sample but 2 M NaOH added into samples before initiation of the reactions; blank II: as enzyme-catalyzed sample but in the absence of enzyme.

  • b 2 M NaOH was added into the wells that did not contain it.