Fold difference between enzyme-catalyzed and blank samples
Blank I
2.4
3.5
Blank II
11.9
17.9
↵a Blank I: as enzyme-catalyzed sample but 2 M NaOH added into samples before initiation of the reactions; blank II: as enzyme-catalyzed sample but in the absence of enzyme.
↵b 2 M NaOH was added into the wells that did not contain it.