Kinetic parameters for norverapamil formation in hepatic or intestinal microsomes of control and diabetic rats
n = 4. Subscripts 1 and 2 denote the high- and low-affinity components of the reaction, respectively.
| Parameters | Control Rats | Diabetic Rats |
|---|---|---|
| Hepatic microsomes | ||
| Vmax (nmol/min per mg of protein) | 1.60 ± 0.30 | 2.38 ± 0.32* |
| Km (μM) | 13.21 ± 1.97 | 16.09 ± 2.32 |
| CLint (ml/min per mg of protein) | 0.122 ± 0.016 | 0.149 ± 0.015* |
| CLint (ml/min per g of liver) | 1.110 ± 0.273 | 1.261 ± 0.206 |
| Intestinal microsomes | ||
| Vmax, 1 (pmol/min per mg of protein) | 49.04 ± 15.78 | 22.70 ± 5.07* |
| Km, 1 (μM) | 34.06 ± 5.19 | 55.37 ± 22.22 |
| CLint, 1 (μl/min per mg of protein) | 1.42 ± 0.28 | 0.43 ± 0.08** |
| Vmax, 2 (pmol/min per mg of protein) | 122.41 ± 32.04 | 139.23 ± 10.75 |
| Km, 2 (μM) | 1041.37 ± 88.47 | 1388.66 ± 157.69* |
| CLint, 2 (μl/min per mg of protein) | 0.12 ± 0.04 | 0.10 ± 0.02 |
| Total CLint (μl/min per mg of protein) | 1.54 ± 0.24 | 0.53 ± 0.10** |
| Total CLint (μl/min per g of intestinal mucosa) | 5.382 ± 1.106 | 1.130 ± 0.235** |