TABLE 4

Kinetic parameters of M28 and M31 formation

Incubation time = 60 min. Protein concentration for incubation with SCH 534128 = 0.8 mg/ml. Protein concentration for incubation with SCH 534129 = 0.4 mg/ml.

Kinetic Parameters of M28 and M31 Formation from Boceprevir with Human Liver Cytosol (1.6 mg protein/ml)^{^a}			Kinetic Parameters of M28 and M31 Formation from SCH 534128 and SCH 534129 with Human Liver Cytosol^{^b}
Parameters	M28	M31	Parameters	M28 from SCH 534128	M31 from SCH 534129
Km (μM)	13.0 ± 4.7	12.5 ± 3.2	K_m (μM)	35.5 ± 9.5	27.4 ± 4.6
V_max (pmol/mg per min)	28.6 ± 5.2	34.9 ± 4.5	V_max (nmol/mg per min)	0.361 ± 0.04	0.846 ± 0.06
V_max/K_m (μl/mg per min)	2.2	2.8	V_max/K_m (μl/mg per min)	10.2	30.9

↵a Samples were analyzed by LC-MS/MS.
↵b Samples were analyzed by HPLC-FSA.