Kinetic parameters of M28 formation from boceprevir with human recombinant AKR1C2 and AKR1C3 enzymes
Intrinsic clearance = Vmax/Km. Samples were analyzed by HPLC-FSA. Protein concentration for AKR1C2 = 2.5 mg/ml and for AKR1C3 = 0.25 mg/ml. Incubation time = 120 min.