Amount and calculated concentration of isoflurane in mouse gallbladder
Isoflurane content of mouse gallbladder bile was measured by LC-MS. See Liquid Chromatography-Mass Spectrometry for details.
| Gallbladder Weight | Gallbladder Isoflurane | Calculated Isoflurane Conc.a | |
|---|---|---|---|
| mg | μg | mM | |
| No isoflurane control | 4.9 | N.D. | N.D. |
| Emptied gallbladder control | 3.3 | N.D. | N.D. |
| Isoflurane-treated mouse 1 | 26.0 | 4.72 | 2.2 |
| Isoflurane-treated mouse 2 | 12.6 | 3.25 | 1.3 |
| Isoflurane-treated mouse 3 | 17.3 | 3.52 | 1.4 |
N.D., not detected.
↵a The calculated isoflurane concentration assumes mouse gallbladder volume of 8 μl/16 g mouse total body weight (Graewin et al., 2004).