Enzyme kinetic parameters of recombinant P450 and UGT enzymes toward AR-67 lactone
Data are means ± S.E.
| Enzyme | Km | Vmax | Ksi |
|---|---|---|---|
| μM | peak area/min/pmol P450/1000 | μM | |
| CYP1A1 | 0.098 ± 0.016 | 48 ± 1.5 | N.A. |
| CYP3A4 | 0.63 ± 0.11 | 622 ± 63 | 3.0 ± 0.58 |
| CYP3A5 | 0.21 ± 0.053 | 275 ± 33 | 2.0 ± 0.56 |
| peak area/min/mg protein/1000 | |||
| UGT1A7 | 0.19 ± 0.026 | 6.6 ± 0.21 | N.A. |
| UGT1A8 | 1.0 ± 0.43 | 48 ± 15 | 1.2 ± 0.58 |
N.A., not applicable.