Percentage extraction recovery and percentage matrix effect of corticosteroids in bovine sclera and choroid-RPE
Percentage extraction recovery was calculated as the ratio of analyte peak area obtained with spiking before extraction to the analyte peak area obtained with spiking postextraction multiplied by 100. Percentage matrix effect was calculated as the ratio of analyte peak area of standard with spiking after extraction procedure to analyte peak area of corresponding unextracted standard multiplied by 100. Data are expressed as the mean ± S.D. for n = 5.
| Corticosteroid Used at 400 ng/ml | Sclera | Choroid-RPE | ||
|---|---|---|---|---|
| % ER | % ME | % ER | % ME | |
| Triamcinolone | 93.22 ± 5.38 | 89.33 ± 5.40 | 90.72 ± 4.40 | 83.04 ± 2.93 |
| Prednisolone | 95.51 ± 6.80 | 94.60 ± 4.37 | 99.12 ± 4.60 | 82.11 ± 2.20 |
| Dexamethasone | 103.44 ± 7.21 | 87.42 ± 6.23 | 86.63 ± 8.75 | 83.72 ± 2.95 |
| Fluocinolone acetonide | 98.09 ± 6.34 | 91.45 ± 8.72 | 93.82 ± 11.81 | 88.20 ± 6.04 |
| Triamcinolone acetonide | 109.32 ± 2.71 | 89.63 ± 3.74 | 90.22 ± 1.61 | 81.83 ± 9.84 |
| Budesonide | 96.56 ± 3.42 | 93.41 ± 5.70 | 86.80 ± 2.73 | 87.24 ± 2.53 |
ER, extraction recovery; ME, matrix effect.