Kinetic parameters of pilocarpine hydrolase activities by HLM and human plasma
Data are mean ± S.D. (n = 3).
| Enzyme Source | 1 mM CaCl2 | Km | Vmax | CLint | Vmax | CLint |
|---|---|---|---|---|---|---|
| mM | nmol · min−1 · mg−1 | μl · min−1 · mg−1 | pmol · min−1 · μl−1 | μl · min−1 · μl−1 | ||
| HLM | ||||||
| − | N.D. | N.D. | N.D. | |||
| + | 7.7 ± 1.0 | 1.0 ± 0.0 | 0.12 ± 0.01 | |||
| Human plasma | ||||||
| − | 2.7 ± 0.6 | 61.7 ± 4.1 | 0.02 ± 0.00 | |||
| + | 3.3 ± 0.8 | 134.5 ± 10.2** | 0.04 ± 0.01* |
N.D., not detected.
↵* P < 0.05 and ** P < 0.001 compared with 1 mM CaCl2 (−).