TABLE 5

Comparison of CYP1A2 and CYP3A4 activity and mRNA levels in hepatocytes treated with glycosylated (HEK293-expressed) or nonglycosylated (E. coli-expressed) IL-6

Donor Hu1190 was treated with IL-6 for 72 h. Data were normalized to untreated values. P values were calculated using a two-tailed, unpaired t test.

P450 IsoformEC50 ValueP
E. coli-Expressed IL-6HEK293-Expressed IL-6
pg/ml
Activity
    1A25756930.76
    3A478.124.30.72
mRNA
    1A1N.D.N.D.N.D.
    1A26165650.77
    2B61.5721.10.32
    2C82712690.18
    2C93032570.19
    2C1977.162.40.48
    2D6N.D.N.D.N.D.
    3A410288.60.27
    3A51932700.55
    4A1111295.70.61

  • N.D., not determined because of a low observed decrease in mRNA levels.