TABLE 4

Donor variability and inducibility of acetaminophen and 6β-hydroxytestosterone formation in IL-6-treated fresh and cryopreserved hepatocytes

All donors were treated for 72 h with IL-6, whereas donor Hu4151 was treated with and without a fixed amount of inducer at each concentration of IL-6. Data were normalized to untreated values. Donors Hu1001, 4151, and 8064 are cryopreserved hepatocytes and Hu1146 and 1150 are fresh hepatocytes.

DonorEC50EmaxEminHill SlopeR2
pg/ml% treated cells
Acetaminophen formation
    Hu100114291.423.0N.A.0.91
    Hu8064407089.852.9−5.970.79
    Hu114626299.36.22−0.690.97
    Hu1150105078.123.7−6.160.97
    Hu415173089.59.33−1.580.98
    Hu4151 + 50 μM omeprazole1120850136−1.420.97
6β-Hydroxytestosterone formation
    Hu10014.2311141.7−0.640.88
    Hu806417698.321.1−0.940.96
    Hu114659.789.610.7−0.880.97
    Hu115043.186.629.7−1.160.93
    Hu415182.881.217.1−1.950.99
    Hu4151 + 10 μM rifampicin157437438.3−1.230.98
  • N.A., not applicable, data fit to fixed-slope dose-response model.