TABLE 4

In vitro anaerobic metabolism of ellagic acid to urolithins in fecal suspensions from four donors

Quantities of substate and products detected in fecal samples taken 0, 6, 24, and 48 h after incubation of ellagic acid at a concentration 50 μM. Data are expressed as mean values of individual donors ± S.E. (n = 3).

Compounds0 h2 h5 h24 h48 h72 h
μM
I
    Ellagic acid18 ± 012 ± 06.5 ± 0.0N.D.N.D.N.D.
    Urolithin CN.D.N.D.0.5 ± 0.00.4 ± 0.0N.D.N.D.
    Isourolithin AN.D.N.D.N.D.8.5 ± 0.310 ± 0.112 ± 0.2
    Urolithin AN.D.N.D.N.D.3.9 ± 0.44.9 ± 0.25.6 ± 0.1
    Urolithin BN.D.N.D.N.D.N.D.N.D.N.D.
        Total urolithinsN.D.N.D.0.5 ± 0.013 ± 0.715 ± 018 ± 0
II
    Ellagic acid23 ± 211 ± 010 ± 01.6 ± 0.21.3 ± 0.11.0 ± 0.2
    Urolithin CN.D.N.D.0.7 ± 0.016 ± 011 ± 01.1 ± 0.0
    Isourolithin AN.D.N.D.N.D.N.D.N.D.0.9 ± 0.0
    Urolithin AN.D.N.D.N.D.0.6 ± 0.012 ± 0.323 ± 1
    Urolithin BN.D.N.D.N.D.N.D.N.D.N.D.
        Total urolithinsN.D.N.D.0.7 ± 0.017 ± 023 ± 125 ± 1
    Ellagic acid32 ± 621 ± 09.2 ± 0.91.0 ± 0.10.6 ± 0.10.5 ± 0.0
    Urolithin CN.D.N.D.0.9 ± 0.014 ± 0.39.0 ± 0.16.7 ± 0.1
    Isourolithin AN.D.N.D.N.D.N.D.N.D.N.D.
    Urolithin AN.D.N.D.N.D.2.9 ± 0.114 ± 015 ± 0
    Urolithin BN.D.N.D.N.D.N.D.N.D.N.D.
        Total urolithinsN.D.N.D.0.9 ± 0.017 ± 023 ± 022 ± 0
IV
    Ellagic acid40 ± 130 ± 013 ± 0.70.9 ± 0.2N.D.N.D.
    Urolithin CN.D.N.D.0.3 ± 0.08.3 ± 0.30.1 ± 0.0N.D.
    Isourolithin AN.D.N.D.N.D.6.6 ± 0.215 ± 0.013 ± 0.0
    Urolithin AN.D.N.D.N.D.4.1 ± 0.27.8 ± 0.28.2 ± 0.1
    Urolithin BN.D.N.D.N.D.N.D.0.8 ± 0.21.7 ± 0.1
        Total urolithinsN.D.N.D.0.3 ± 0.019 ± 0.723 ± 123 ± 0.3

  • N.D., not detected.