Unbound and covalently bound radioactivity in plasma samples measured by size exclusion chromatography after single oral administration of [14C]GSK977779 (30 mg/kg) to male and female rats
| Time Postdose | Sex | Total Drug-Related Materiala | %Unboundb | %Covalently Boundc | Total Recoveryd |
|---|---|---|---|---|---|
| h | μg eq/g | ||||
| 2 | M | 211 | 95.7 | 1.2 | 96.9 |
| 4 | M | 157 | 93.8 | 2.4 | 96.2 |
| 8 | M | 84.0 | 87.6 | 8.5 | 96.2 |
| 24 | M | 14.0 | 62.6 | 30.8 | 93.4 |
| 2 | F | 226 | 72.5 | 23.8 | 96.3 |
| 4 | F | 149 | 41.0 | 55.9 | 96.9 |
| 8 | F | 90.0 | 8.5 | 86.9 | 95.4 |
| 24 | F | 44.0 | 2.8 | 96.0 | 98.8 |
M, male; F, female.
a Mean concentration of radioactivity (n = 3) in plasma of males and females. All other data were obtained from pooled samples per sex across time points.
b Amount of radioactivity recovered in chromatographic region where small molecules eluted.
c Amount of radioactivity recovered in chromatographic region where albumin standard eluted.
d The sum of the percentage unbound and percentage covalently bound radioactivity.