Extract | RDI | UGT1A4 | UGT1A6 | UGT1A9 | |||
---|---|---|---|---|---|---|---|
Rough IC50 | VDI | Rough IC50 | VDI | Rough IC50 | VDI | ||
mg | μg/ml | l/dose | μg/ml | l/dose | μg/ml | l/dose | |
Black cohosh | 40 | 69.7 ± 4.8 | 0.6 | N.A. | N.A. | 321.6 ± 102.2 | 0.1 |
Cranberry | 1000 | 742.7 ± 118.7 | 1.3 | >1000 | <1.0 | 260.5 ± 33.0 | 3.8a |
Echinacea | 400 | 116.1 ± 25.1 | 3.4 | 241.0 ± 23.4 | 1.7 | 858.3 ± 158.7 | 0.5 |
Garlic | 1000 | N.A. | N.A. | N.A. | N.A. | N.A. | N.A. |
Ginkgo biloba | 240 | 268.2 ± 48.9 | 0.9 | N.A. | N.A. | PBb | PB |
Acid-hydrolyzed Ginkgo biloba | 240 | Interfc | N.A. | N.A. | PB | PB | |
Ginseng | 550 | 368.4 ± 66.6 | 1.5 | N.A. | N.A. | 298.6 ± 29.1 | 1.8 |
Acid-hydrolyzed ginseng | 550 | 288.0 ± 42.8 | 1.9 | >1000 | <0.6 | 524.3 ± 60.5 | 1.0 |
Milk thistle | 600 | Interf | 66.9 ± 3.5 | 9.0a | 35.9 ± 4.3 | 16.7a | |
Saw palmetto | 320 | 70.6 ± 9.3 | 4.5 | 131.8 ± 21.5 | 2.4a | N.A. | N.A. |
Valerian | 1000 | 406.5 ± 35.3 | 2.5 | >1000 | <1.0 | N.A. | N.A. |
EGCG | 250 | 34.39 ± 4.1 | 7.3a | 183.6 ± 29.8 | 1.4 | N.A. | N.A. |
N.A., data points did not fit the IC50 curve.
a Volume/dose index values that exceed the cutoff for further investigation.
b PB, IC50 values for inhibition of UGT1A9 by ginkgo and acid-hydrolyzed ginkgo extracts have been reported previously (Mohamed and Frye, 2010). Ginkgo and acid-hydrolyzed ginkgo extracts inhibited MPAG formation in HLM with IC50 values of 84.3 ± 11.6 and 20.9 ± 3.6 μg/ml, respectively. For a dose of 240 mg, this would result in VDI of 2.9 and 11.4 l/dose for unhydrolyzed and acid-hydrolyzed ginkgo extracts, respectively.
c Interf: addition of herb interfered with florescence detection of glucuronide.