Apparent kinetic parameters of eslicarbazepine glucuronidation in HLM and recombinant UGT enzymes
Values represent mean ± S.E.M. of duplicate samples.
| Km (μM) | Vmax (peak area ratio/mg protein per minute) | |||
|---|---|---|---|---|
| No BSA | 2% BSA | No BSA (×104) | 2% BSA (×104) | |
| HLM | 412.2 ± 63.8 | 349.7 ± 74.3 | 27.1 ± 1.8 | 98.7 ± 8.7 |
| UGT1A9 | 1299.0 ± 841.8 | 577.1 ± 330.1 | 43.0 ± 1.8 | 48.0 ± 1.3 |
| UGT2B4 | 157.0 ± 31.2 | 28.7 ± 10.1 | 1.7 ± 0.2 | 0.9 ± 0.07 |
| UGT2B17 | 524.2 ± 195.9 | 279.3 ± 62.9 | 1.5 ± 0.25 | 1.4 ± 0.2 |