Step | Volume | Total Protein | Benzaldehyde/DCPIP Activity | |||
---|---|---|---|---|---|---|
Total Activity | Specific Enzyme Activity | Yield | Fold Purification | |||
ml | mg | IU | IU/mg | % | ||
Crude extract | 280 | 17,513 | 163.3 | 0.009 | 77 | 0.8 |
Cleared lysate | 280 | 18,354 | 212.9 | 0.012 | 100 | 1.0 |
Flow-through | 280 | 18,597 | 170.8 | 0.009 | 80 | 0.8 |
Ni-NTA | 30.4 | 35 | 3.6 | 0.104 | 1.71 | 9.0 |
Chemical sulfuration | 49.4 | 32 | 5.0 | 0.158 | 2.34 | 13.6 |
Superose 6 | 15.2 | 12 | 4.9 | 0.414 | 2.30 | 35.7 |