TABLE 4

Steady-state kinetic parameters of recombinant and native mAOX3, with different aldehyde and purine substrates

Apparent kinetic parameters were recorded in 100 mM potassium phosphate (pH 7.4) by varying the concentration of substrate in the presence of 1 mM ferricyanide as an electron acceptor.

Native mAOX3Recombinant mAOX3
kcatKMkcatKM
l/minμMl/minμM
Benzaldehyde130 ± 813 ± 644 ± 220 ± 6
Butyraldehyde384 ± 4029 ± 8140 ± 1026 ± 5
2-OH-pyrimidine1279 ± 55173 ± 18413 ± 1697 ± 11