Kinetic parameters of AM demethylation by CYP2B6 proteins expressed in COS-7 cells
Results represent the mean ± S.D. of triplicate determinations.
| Variants | Apparent Km | Vmax | Vmax/Apparent Km | Vmax/Apparent Km Ratio |
|---|---|---|---|---|
| μM | pmol · min−1 · pmol CYP2B6−1 | μl · min−1 · pmol CYP2B6−1 | % CYP2B6.1 | |
| CYP2B6.1 | 3.10 ± 1.1 | 36.0 ± 5.67 | 12.4 ± 4.11 | |
| CYP2B6.2 | 4.29 ± 2.7 | 64.4 ± 3.92* | 18.8 ± 9.19 | 129 |
| CYP2B6.4 | 2.73 ± 0.45 | 70.6 ± 9.29* | 26.0 ± 2.42 | 223 |
| CYP2B6.5 | 6.87 ± 6.8 | 19.8 ± 3.06 | 8.91 ± 11.3 | 24.8 |
| CYP2B6.6 | 6.72 ± 3.0 | 150 ± 15.9* | 24.2 ± 6.84 | 192 |
| CYP2B6.7 | 2.80 ± 1.4 | 50.1 ± 12.3 | 19.2 ± 4.65 | 154 |
| CYP2B6.9 | 4.44 ± 1.7 | 33.1 ± 5.20 | 8.38 ± 3.94 | 64.2 |
| CYP2B6.10 | 1.93 ± 0.68 | 17.0 ± 5.03 | 9.98 ± 5.05 | 75.7 |
| CYP2B6.13 | 7.33 ± 4.1 | 18.2 ± 5.54 | 2.93 ± 1.73 | 21.3 |
| CYP2B6.14 | 5.06 ± 5.8 | 7.06 ± 1.63* | 6.70 ± 9.40 | 12.0 |
| CYP2B6.17 | 2.17 ± 0.40 | 21.2 ± 4.73 | 9.44 ± 2.45 | 84.0 |
| CYP2B6.19 | 8.06 ± 8.9 | 36.9 ± 21.9 | 7.38 ± 6.15 | 39.4 |
| CYP2B6.20 | 6.47 ± 9.8 | 9.85 ± 2.22* | 8.79 ± 7.22 | 13.1 |
| CYP2B6.23 | 1.91 ± 0.72 | 31.4 ± 7.71 | 17.6 ± 5.93 | 142 |
| CYP2B6.25 | 2.04 ± 1.9 | 25.7 ± 7.31 | 21.6 ± 15.7 | 108 |
| CYP2B6.26 | 5.50 ± 3.4 | 37.3 ± 6.04 | 10.1 ± 8.13 | 58.4 |
| CYP2B6.27 | 4.50 ± 0.98 | 10.6 ± 6.16* | 2.59 ± 1.82 | 20.2 |
* P < 0.05 compared with CYP2B6.1.