Oligonucleotide | 5′ to 3′ Sequence |
---|---|
For construction of cosmid DNA | |
S-PGRMC1 | GAGTTCCGGATCCCTGCCa |
AS-PGRMC1 | ATACCTCGAGAGATATACTTCCACTGb |
S-CYP2E1-1 | ATGTCTGCCCTCGGAGTCAC |
AS-CYP2E1-1 | CTCATGAGCGGGGAATGACA |
For construction of FLAG-tagged PGRMC1 plasmid | |
S-PGRMC1 | See above sequence |
AS-FLAG PGRMC1 | TAGACTCGAGCTACTTGTCATCGTCATCCTTGTAGTCATCATTTTTCCGGGCACTCd |
For construction of Myc-tagged P450 plasmids | |
S-CYP3A4 | TCACTCGAGATGGCTCTCATCCCAGACTTGb,c |
AS-CYP3A4 | CTCGGTACČGGCTCCACTTACGGTGCCATCe |
S-CYP2C9 | TCACTCGAGATGGATTCTCTTGTGGTCb,c |
AS-CYP2C9 | CTCGGTACČGACAGGAATGAAGCACAGCe |
S-CYP2E1-2 | TCACTCGAGATGTCTGCCCTCGGAGTCACb,c |
AS-CYP2E1-2 | CTCGGTACČTGAGCGGGGAATGACACAGAGe |
S-3×Myc | CTCGGTACCGAGCAGAAGCTGATCAGCGAGGAGGACCTGGAGCAGAAGCTGATCAGCGAGGAGGACCTGGAGCAGAAGCTGATCAGCGAGGAGGACCTGTGAGCGGCCGCGTCGACAGf |
AS-3×Myc | CTGTCGACGCGGCCGCTCACAGGTCCTCCTCGCTGATCAGCTTCTGCTCCAGGTCCTCCTCGCTGATCAGCTTCTGCTCCAGGTCCTCCTCGCTGATCAGCTTCTGCTCGGTACCGAGg |
S, sense; AS, antisense.
↵a The BamHI site is underlined.
↵b The XhoI site is underlined.
↵c The start codon is in bold.
↵d The XhoI site is underlined. Complementary sequences of FLAG tag are in bold. Complementary sequences of stop codon (TAG) are italicized.
↵e The KpnI site is underlined. The deleted stop codon is shown with the inverted carat.
↵f The KpnI and NotI sites are underlined. Three tandem Myc tags are in bold. The stop codon (TGA) is italicized.
↵g The NotI and KpnI sites are underlined. Complementary sequences of three tandem Myc tags are in bold. Complementary sequences of stop codon (TGA) are italicized.