Details of in vitro P450 inhibition assay conditions and positive control inhibitors
The microsomal protein concentration in each assay was standardized at 0.1 mg/ml.
| Substrate | [Substrate] | Incubation Time | Positive Control | |
|---|---|---|---|---|
| μM | min | |||
| CYP1A2 | Phenacetin | 35 | 10 | Fluvoxamine furafyllinea |
| CYP2C8 | Paclitaxel | 10 | 10 | Quercetin phenelzinea |
| CYP2C9 | Diclofenac | 8 | 5 | Sulphaphenazole tienilic acida |
| CYP2C19 | S-Mephenytoin | 50 | 10 | Ticlopidineb |
| CYP2D6 | Bufuralol | 5 | 10 | Quinidine paroxetinea |
| CYP3A4 | Atorvastatin | 50 | 10 | Ketoconazole troleandomycina |
| Midazolam | 2.5 | 5 | ||
| Nifedipine | 10 | 10 |