Calculated AUC of genistein, daidzein, and derived metabolites after intragastric genistein and/or daidzein administration
| Isoflavone | Aglycone or Metabolite | Wild-Type | Knockout AUC | pa |
|---|---|---|---|---|
| AUC h nM | AUC h nM | |||
| Genistein (50 mg/kg) | 254.7 ± 23.9 | 371.2 ± 31.1 | 0.097 | |
| Gen-sulfate | 326.9 ± 92.2 | 2,338.8 ± 224.8 | 0.014 | |
| Gen-gluc 1 | 241.2 ± 69.5 | 1,992.3 ± 242.5 | 0.02 | |
| Gen-gluc 2 | 47.5 ± 13.1 | 99.9 ± 14.3 | 0.14 | |
| Gen-gluc 3 | 44.8 ± 10.8 | 106.1 ± 17.7 | 0.097 | |
| Total | 900.9 ± 207.3 | 4,987.7 ± 508.4 | 0.018 | |
| Daidzein (50 mg/kg) | Daidzein | 222.43 ± 32.5 | 528.9 ± 80.2 | 0.071 |
| Daid-sulfate | 361.4 ± 67.0 | 2,459.1 ± 365.1 | 0.029 | |
| Daid-gluc 1 | n.d. | 977.2 ± 196.0 | ||
| Daid-gluc 2 | n.d. | n.d. | ||
| Total | 583.7 ± 89.7 | 4,012.1 ± 611.7 | 0.031 | |
| Genistein+daidzein (25 + 25 mg/kg) | Genistein | 149.4 ± 12.8 | 251.5 ± 27.8 | 0.079 |
| Gen-sulfate | 284.7 ± 32.4 | 1,765 ± 145.5 | 0.0099 | |
| Gen-gluc 1 | 162.9 ± 33.6 | 1,155.0 ± 192.6 | 0.0366 | |
| Daidzein | 157.8 ± 26.3 | 355.3 ± 51.3 | 0.0756 | |
| Daid-sulfate | 171.5 ± 45.9 | 1,687.0 ± 307.6 | 0.039 | |
| Total | 926.4 ± 138.9 | 5,174.5 ± 696.1 | 0.026 |
n.d., not detected; Gen-gluc 2, genistein glucuronide isomer 2; Gen-gluc 3, genistein glucuronide isomer 3; Daid-gluc 1, daidzein glucuronide isomer 1; Daid-gluc 2, daidzein glucuronide 2.
↵a Bold text indicates significant differences (p < 0.05).