AdCYP3A4 + AdPGRMC1 | Testosterone 6β-Hydroxylation | Midazolam 1′-Hydroxylation | |||||
---|---|---|---|---|---|---|---|
Km | Vmax | Vmax/Km | Km | Vmax | Ki | Vmax/Km | |
μM | pmol · min−1 · pmol P450−1 | μl · min−1 · pmol P450−1 | μM | pmol · min−1 · pmol P450−1 | μM | μl · min−1 · pmol P450−1 | |
56.9 ± 9.1 | 54.3 ± 4.0 | 0.96 ± 0.08 | 5.7 ± 0.9 | 9.8 ± 1.5 | 15.9 ± 4.1 | 1.72 ± 0.03 | |
×1 | 53.1 ± 22.3 | 51.2 ± 5.9 | 1.07 ± 0.42 | 10.9 ± 2.2* | 6.5 ± 0.7* | 10.4 ± 7.5 | 0.60 ± 0.05* |
×2 | 71.4 ± 14.2 | 43.4 ± 4.4* | 0.62 ± 0.07* | 11.6 ± 3.8* | 5.0 ± 1.4* | 17.8 ± 11.6 | 0.44 ± 0.04* |
×4 | 91.8 ± 12.2* | 37.4 ± 2.7** | 0.41 ± 0.04* | 12.5 ± 2.1* | 4.1 ± 0.6** | 16.3 ± 3.1 | 0.33 ± 0.01** |
↵* P < 0.05 and ** P < 0.01 compared with control by Shirley-Williams' test.