Effect of the GST isoforms on the increase in the formation of Met4
Relative peak was calculated by eq. 1, as mentioned under Materials and Methods. Statistical analysis was performed using a one-way ANOVA followed by Dunnett's test.
| GST Isoforms | Relative Peak Area | |
|---|---|---|
| GST 0.1 mg/ml | GST 0.2 mg/ml | |
| % | ||
| No GST | 100 ± 3 | 100 ± 10 |
| GSTA1 | 111 ± 6 | 129 ± 14** |
| GSTA2 | 114 ± 9 | 95.3 ± 12.4 |
| GSTM1 | 182 ± 15** | 209 ± 22** |
| GSTT1 | 89.4 ± 15.8 | 104 ± 15 |
| GSTP1 | 123 ± 8** | 141 ± 13** |
↵** , p < 0.01, significant difference (incubation sample in the absence of GSTs versus incubation sample in the presence of each GST isoform). Each value represents the mean ± S.E. (n = 6).