TABLE 1

Sequence specificity ABC transporter subfamily B

The selected proteotypic peptide, AGAVAEEVLAAIR, for human P-gp is unique and distinct from other human transporters from the ABCB subfamily; different amino acids are depicted in bold italic type. The sequence is specific for an ABCB1 region that is highly conserved between species and found to be identical in abcb1a and abcb1b isoforms in mouse and rat and abcb1 in dog and human.

ABCB Protein and SpeciesSequence Agreement Proteotypic Peptide
ABCB1_HUMANAGAVAEEVLAAIR
ABCB1a_RATAGAVAEEVLAAIR
ABCB1b_RATAGAVAEEVLAAIR
ABCB1a_MOUSEAGAVAEEVLAAIR
ABCB1b_MOUSEAGAVAEEVLAAIR
ABCB1_DOGAGAVAEEVLAAIR
ABCB4_HUMANAGAVAEEALGAIR
ABCB11_HUMANAGVVADEVISSMR
ABCB5_HUMANN.A.
ABCB2_HUMANN.A.
ABCB3_HUMANN.A.
ABCB9_HUMANN.A.
ABCB8_HUMANN.A.
ABCB10_HUMANN.A.
ABCB6_HUMANN.A.
ABCB7_HUMANN.A.

  • N.A., no sequence homology to identified ABCB1 tryptic digestion fragment.