Intraday and interday validation of P-gp synthetic proteotypic peptide quantification
The synthetic proteotypic peptide was spiked to the membrane fraction of a membrane vesicle preparation originating from untransfected HEK293 cells (matrix) at two concentrations (100 and 1000 pmol/l). Preparations were performed at three independent occasions.
| Experiment | Matrix | 100 pM | 1000 pM |
|---|---|---|---|
| Day 1 | |||
| n | 3 | 3 | |
| Mean | 50.8 | 91.2 | 1017.6 |
| Accuracy (% RE) | −8.8 | 1.8 | |
| Precision (% CV) | 9.6 | 10.9 | |
| Day 2 | |||
| n | 3 | 3 | |
| Mean | 41.6 | 113.1 | 935.7 |
| Accuracy (% RE) | 13.1 | −6.4 | |
| Precision (% CV) | 12.1 | 8.5 | |
| Day 3 | |||
| n | 3 | 3 | |
| Mean | 49.1 | 87.4 | 918.5 |
| Accuracy (% RE) | −12.6 | −8.1 | |
| Precision (% CV) | 11.3 | 10.2 | |
| Interday | |||
| n | 9 | 9 | |
| Mean | 97.2 | 957.3 | |
| Accuracy (% RE) | −2.8 | −4.2 | |
| Precision (% CV) | 12.0 | 10.7 |
%RE, percentage relative error; %CV, coefficient of variation.