Predicted b and y ion series for the modified peptide sequence 299GTETTSTTLR308 with MH+ at m/z 1240.7 for adduct formation by BPA at the Thr302a
| b Ions | m/z | Amino Acid Sequence | y Ions | m/z |
|---|---|---|---|---|
| b1 | G | y10 | ||
| b2 | 159.2 | T | y9 | 1184.1 |
| b3 | 288.3 | E | y8 | 1082.9 |
| b4 | 563.4 | T + BPA + oxygen | y7 | 953.9 |
| b5 | 664.5 | T | y6 | 678.8 |
| b6 | 751.6 | S | y5 | 577.7 |
| b7 | 852.7 | T | y4 | 490.6 |
| b8 | 953.8 | T | y3 | 389.5 |
| b9 | 1066.9 | L | y2 | 288.4 |
| b10 | R | y1 | 175.2 |
a The b and y ion series for the Thr302-modified peptide with an MH+ ion at m/z 1240.7 were calculated from the theoretical b and y ions for the unmodified peptide with the increase of 174 Da using the ProteinProspector software.