TABLE 2

Amount of chlorogenic acids transported by gastric epithelial monolayers and associated log D and permeability coefficients

Cultures were incubated (40 min) in the presence of coffee solution containing 1445 nmol of total CGAs (see Table 1). Apical, pH 3 (0.5 ml); basal, pH 7.4 (1.5 ml). Total recovery of CGAs was 91%; CGAs with no observed permeation were not presented. The permeability coefficient is expressed as ×10⁻⁶ cm/s ± S.D., mean of three culture samples. Values in italics are initial (at 10 min) permeability measurements. %Apical dose, values in parentheses indicate the amount of each individual CGA expressed as a percentage of the total CGA content.

Compound	Amount Detected in Transport Wells^{^a}				Permeability Coefficient (P_app) and Log D
	Amount Detected in Transport Wells^{^a}				Gastric Monolayer Permeability		Caco-2 Monolayer Permeability
	Apical		Basal		P_app	Log D_{pH 3}	P_app	Log D_{pH 7.4}
	% of Apical Dose
3-O-caffeoylquinic acid	208.4 ± 6.3	(14.4)	0.2 ± 0.1	(0.02)	0.15 ± 0.01	−0.43	0.1 ± 0.01	−3.58
4-O-caffeoylquinic acid	309.6 ± 6.0	(21.4)	0.4 ± 0.1	(0.03)	0.15 ± 0.01	−0.42	0.13 ± 0.03	−3.57
5-O-caffeoylquinic acid	333 ± 9.5	(23)	0.3 ± 0.1	(0.02)	0.08 ± 0.003	−0.43	0.04 ± 0.003	−3.58
3-O-feruloylquinic acid	46 ± 1.9	(3.2)	0.04 ± 0.01	^{^b}	0.14 ± 0.01	−0.29	0.21 ± 0.06	−3.42
4-O-feruloylquinic acid	49.8 ± 1.2	(3.4)	0.05 ± 0.02	^{^b}	0.11 ± 0.01	−0.27	0.14 ± 0.05	−3.43
5-O-feruloylquinic acid	95.4 ± 2.2	(6.6)	0.07 ± 0.02	^{^b}	0.06 ± 0.003	−0.29	0.05 ± 0.01	−3.43
3-O-caffeoylquinic acid lactone	57.6 ± 1.0	(4.0)	0.13 ± 0.05	(0.01)	0.19 ± 0.01	0.40	0.25 ± 0.05	0.39
4-O-caffeoylquinic acid lactone	63.5 ± 1.2	(4.4)	0.12 ± 0.04	(0.01)	0.15 ± 0.02	0.40	0.31 ± 0.05	0.39
3,4-di-O-caffeoylquinic acid	45.6 ± 2.6	(3.2)	0.04 ± 0.01	^{^b}	2.08 ± 0.8	1.99	1.16 ± 0.2	−1.16
3,5-di-O-caffeoylquinic acid	16.1 ± 1.8	(1.1)	0.12 ± 0.01	(0.01)	9.83 ± 1.1	1.97	N.D	−1.17
4,5-di-O-caffeoylquinic acid	31.3 ± 2.2	(2.2)	0.02 ± 0.003	^{^b}	2.62 ± 0.8	1.99	1.77 ± 0.4	−1.16
Total chlorogenic acids	1256 ± 35.4	(86.9)	1.5 ± 0.5	(0.1)

n.d., not detected.
↵a Values are expressed as amount (nanomoles) ± S.D. (n = 3).
↵b Less than 0.01% of the total CGA content.