TABLE 3

Correlation between protein and mRNA expression levels of drug-metabolizing enzymes

Least-squares regression analysis was performed for protein expression levels (x-values, pmol/mg protein) against mRNA expression levels (y-values, relative amount to β-actin). Data points are shown in Fig. 2.

Enzyme1/Sloper2
High correlation
    CYP3A445.50.944
    CYP2B63.750.702
    CYP2C826.20.649
Medium correlation
    CYP2C1945.70.586
    CYP2D61510.521
    CYP3A757.10.487
    CYP3A584.90.441
Low correlation
    CYP1A258.00.163
    CYP2C972.00.114
    CYP2A6<0.100
    CYP2E1<0.100
    CYP4A11<0.100
    UGT1A65500.622
    UGT1A91060.309
    UGT2B758.50.282
    UGT2B159050.250
    UGT1A1<0.100

  • 1/Slope, reciprocal slope of regression line; r2, correlation coefficient of regression line.