Kinetic parameters of 4HCY formation determined in reconstituted enzyme systems
| Enzyme | Amino Acid Change | Kma | Vmaxa | Intrinsic Clearanceb |
|---|---|---|---|---|
| mM | pmol · min−1 · pmol P450−1 | nl · min−1 · pmol P450−1 | ||
| CYP2B6.1 | None | 3.6 ± 0.1 | 75.4 ± 0.9 | 20.7 |
| CYP2B6.4 | K262R | 3.5 ± 0.1 | 50.6 ± 0.6 | 14.4 |
| CYP2B6.5 | R487C | 5.1 ± 0.4 | 54.6 ± 1.5 | 10.8 |
| CYP2B6.6 | K262R/Q172H | 4.0 ± 0.1 | 117 ± 1 | 29.0 |