TABLE 3

Kinetic parameters for OM-hydrolyzing activities of various proteins in human plasma

Data generated in duplicate determinations were fitted to the single-enzyme Michaelis-Menten model by using nonlinear least-squares regression.

Enzyme SourceKmVmaxVmax/Km
μMnmol · min−1 · mg protein−1ml · min−1 · mg protein−1
Plasma6.7118.62.77
Recombinant PON1192QQ1571240.790
Recombinant PON1192RR1021401.37
Serum albumin3542.610.00737