TABLE 1
Comparison of kinetics parameters of UGT1A9 from different sources in genistein and apigenin glucuronidation
A Student's t test was used to compare the data. For Supersomes and transient overexpression of UGT1A9, three independent studies (n = 3) were run, whereas for stable overexpression of UGT1A9, only two independent studies (n = 2) were run. The arrows indicate the position for glucuronidation occurring in the engineered HeLa cells.
| Genistein | Apigenin | |||||
|---|---|---|---|---|---|---|
| Source |  |  | ||||
| Supersomes | Transient | Stable | Supersomes | Transient | Stable | |
| Km (μM) | 2.72 ± 0.073 | 3.71 ± 0.65 | 3.83 ± 0.18* | 0.51 ± 0.048 | 1.76 ± 0.11* | 1.68 ± 0.037 |
| Vmax (nmol · min−1 · mg−1) | 1.41 ± 0.027 | 0.35 ± 0.025 | 0.10 ± 0.0015 | 2.61 ± 0.023 | 1.58 ± 0.0054 | 0.21 ± 0 |
| AIC | −15.82 | −36.63 | −76.62 | −10.44 | −18.96 | −47.84 |
| R2 | 0.97 | 0.99 | 0.99 | 0.91 | 0.86 | 0.92 |
AIC, Akaike information criterion.
↵* p < 0.05.